Research Technician II

SHIFT: Day (United States of America) Seeking Breakthrough Makers Children’s Hospital of Philadelphia (CHOP) offers countless ways to change lives. Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation. At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families. Join us as we build on our promise to advance pediatric care—and your career. CHOP does not discriminate on the basis of race, color, sex, national origin, religion, or any other legally protected categories in any employment, training, or vendor decisions or programs. CHOP recognizes the critical importance of a workforce rich in varied backgrounds and experiences and engages in ongoing efforts to achieve that through equally varied and non-discriminatory means. A Brief Overview This entry-level research technical role participates in a planned research projects by performing research protocols, experimental procedures and other laboratory tasks under direct supervision. May occasionally exercise minimal discretion and judgment in execution and interpretation of experiments, and of data derived. The Wang Laboratory at the Children’s Hospital of Philadelphia (CHOP) is seeking a highly motivated Research Technician II to join our research team. Our laboratory studies the molecular and cellular mechanisms governing heart development, cardiomyocyte maturation, and congenital heart disease. We combine single-cell and spatial genomics, in vivo functional genomics, mouse genetics, genome editing, and human stem cell–based models to identify regulatory mechanisms controlling cardiac development and disease. A major focus of the laboratory is developing and applying new technologies that connect large-scale genomic discovery with functional validation in vivo. The Research Technician will work closely with the principal investigator and other lab members and will have opportunities to contribute directly to ongoing research projects, lab management, learn advanced experimental approaches, and participate in scientific presentations and publications. What you will do The Research Technician will support multiple projects related to heart development and functional genomomics. Responsibilities may include: Perform molecular and cellular biology experiments, including PCR/qPCR, DNA/RNA preparation, molecular cloning, immunofluorescence staining, microscopy, and related assays. Assist with mouse-based studies of cardiac development, including tissue collection, sample processing, genotyping, and maintenance of experimental records. Support AAV- and CRISPR-based in vivo functional genomic experiments. Prepare cardiac tissue and nuclei for single-cell, single-nucleus, and spatial genomic studies. Assist with mammalian cell culture and, depending on project needs and experience, human iPSC culture and differentiation. Carefully document…